Anti-GRIA3 Polyclonal Antibody for WB, IF/ICC, ELISA - P42263

Core Product Specifications and Parameters

Biological Background: GRIA3 Function and Localization

• GRIA3/AMPA receptor 3 is a ligand-gated cation channel that mediates fast excitatory synaptic transmission by gating calcium influx upon L-glutamate binding, leading to rapid desensitization into an inactive state with bound agonist (PubMed:17989220). Related references: PMID:17989220

• Interaction with CACNG8 modulates receptor kinetics, inducing resensitization characterized by a delayed accumulation of current flux during sustained glutamate application (PubMed:21172611). Related references: PMID:21172611

• The receptor is localized to the postsynaptic cell membrane, specifically enriched in the postsynaptic density, where it plays a central role in synaptic plasticity and long-term potentiation (By similarity).

• GRIA3 undergoes extensive post-translational modifications, including glycosylation, disulfide bond formation, phosphorylation, and palmitoylation, which regulate its trafficking, localization, and function.

• Alternative splicing generates multiple isoforms, contributing to functional diversity in synaptic signaling.

• Mutations in GRIA3 are associated with intellectual disability (MRX94/MRXSW), linking its dysfunction to neurodevelopmental disorders.

• As a member of the AMPA receptor family, it shares structural homology with other subunits (GRIA1, GRIA2, GRIA4), and its subunit composition determines channel properties such as calcium permeability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 200–490 of human GRIA3, a region within the extracellular domain. This suggests the antibody may preferentially recognize surface‑exposed or native epitopes, making it potentially suitable for immunocytochemical detection of surface receptors.

• For western blotting, a primary band at approximately 101 kDa is expected; minor bands from alternative splicing or post‑translational modifications may also be observed. Validate specificity using brain lysates or neuronal cell lines (e.g., SH‑SY5Y, primary cortical neurons).

• In immunofluorescence/immunocytochemistry, include appropriate controls (e.g., antigen blocking, knockout cell lines) to confirm specificity. Optimize fixation and permeabilization conditions; paraformaldehyde fixation followed by mild permeabilization with Triton X‑100 is a common starting point.

• For ELISA applications, pair this antibody with a matched monoclonal or other polyclonal antibody raised against a distinct epitope to enable sandwich ELISA development; assess cross‑reactivity with other AMPA receptor subunits (GRIA1, GRIA2, GRIA4) to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this anti-GRIA3 polyclonal antibody, ideal for neuroscience diagnostic assay development. Our supply capabilities include validated antibody pairs, optimized recombinant proteins, and ancillary reagents, providing a one-stop solution for researchers and manufacturers targeting glutamate receptor pathways. Contact us for bulk orders or assistance with rare target material sourcing.