Anti-GRHPR Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UBQ7

Core Product Specifications and Parameters

Biological Background: GRHPR Function and Localization

• GRHPR (Glyoxylate reductase/hydroxypyruvate reductase) is a ubiquitous enzyme predominantly expressed in the liver, catalyzing the reduction of hydroxypyruvate to D-glycerate and glyoxylate to glycolate, as well as the oxidation of D-glycerate to hydroxypyruvate.
• The enzyme belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family, utilizing NADP as a cofactor, and plays a role in glyoxylate metabolism and serine biosynthesis pathway regulation.
• Functional studies indicate that GRHPR activity is critical for detoxifying glyoxylate and preventing oxalate accumulation, with genetic mutations linked to primary hyperoxaluria type II (PH2).
• Keywords from UniProt include: Alternative splicing, NADP, Oxidoreductase, Phosphoprotein, and 3D-structure, suggesting potential structural and regulatory complexity.
• Tissue expression profiling reveals highest levels in the liver, consistent with its central metabolic function in hepatic glyoxylate handling.
• As a phosphoprotein, GRHPR may be subject to post-translational modification that could modulate its enzymatic activity in response to cellular signals.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 36 kDa is expected; use liver tissue lysates or GRHPR-overexpressing cell lines as positive controls given its high expression in liver.
• In immunofluorescence experiments, consider co-staining with organelle markers to investigate subcellular localization, as GRHPR’s cellular compartment remains not fully characterized.
• For ELISA, the polyclonal nature may enhance sensitivity, but titration and cross-reactivity testing against related dehydrogenases is advisable.
• Pre-validation in mouse and rat samples is recommended to confirm expected cross-reactivity before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality raw materials for immunoassay development. Complementing this anti-GRHPR antibody, we offer validated antibody pairs, bulk ancillary reagents, and rare-target sourcing services to support your glyoxylate metabolism studies and IVD assay construction. Contact us to explore customized raw-material solutions from concept to clinic.