Anti-GRB2 Polyclonal Antibody for WB, IF/ICC, ELISA - P62993

Core Product Specifications and Parameters

Biological Background: GRB2 Function and Localization

• GRB2 (Growth factor receptor-bound protein 2) is a non-enzymatic adapter protein that mediates signaling from cell surface receptors to downstream pathways, regulating gene expression, cell proliferation, and differentiation. Related references: PMID:11016927, PMID:11726515, PMID:37626338
• It participates in diverse processes including T-cell and B-cell activation, autophagy, DNA repair, and necroptosis. Related references: PMID:35831301, PMID:37626338, PMID:38182563, PMID:36864087, PMID:9489702
• At the T-cell receptor (TCR), GRB2 binds phosphorylated LAT and recruits SOS1, leading to RAS-MAPK pathway activation. Related references: PMID:9489702, PMID:12171928, PMID:25870599
• In B-cells, GRB2 amplifies Ca²⁺ signaling and ERK activation upon BCR engagement. Related references: PMID:25413232, PMID:29523808
• GRB2 balances autophagy and necroptosis by interacting with RIPK1, SQSTM1, and BECN1 downstream of EGFR. Related references: PMID:35831301, PMID:38182563
• It regulates miRNA processing by forming a complex with AGO2 and DICER1. Related references: PMID:37328606
• GRB2 protects stalled replication forks from MRE11-mediated degradation and facilitates homology-directed repair. Related references: PMID:38459011, PMID:34348893
• An alternatively spliced isoform, Grb3-3, lacks SH3 domain function and acts as a dominant negative inhibitor of GRB2-mediated signaling by competing for SOS1 binding, thereby suppressing cell proliferation. Related references: PMID:36171279
• GRB2 localizes to the nucleus, cytoplasm, endosome, and Golgi apparatus and contains SH2 and SH3 domains that mediate protein–protein interactions.

Experimental Guidance and Technical Tips

• The immunogen spans full-length human GRB2 (aa 1–217); verify detection of the Grb3-3 isoform in your system if relevant.
• For western blot, expect a band around 25 kDa; post-translational modifications may cause slight shifts.
• In IF/ICC, GRB2 may be observed in multiple subcellular compartments; optimize fixation and permeabilization conditions for your cell type.
• Confirm assay specificity using appropriate controls, as the antibody cross-reacts with human and mouse GRB2.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated B2B partner providing one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. This anti-GRB2 antibody is well suited for research in cell signaling, immunology, and oncology. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and can source even rare-target raw materials to support your diagnostic development and manufacturing needs.