Anti-GRB14 Rabbit Polyclonal Antibody for WB, ELISA - Q14449

Core Product Specifications and Parameters

Biological Background: GRB14 Function and Localization

• Protein Identity: GRB14 (Growth factor receptor-bound protein 14) is an adapter protein containing an SH2 domain, playing a key role in modulating intracellular signaling cascades.
• Receptor Interaction: It binds specifically to the activated insulin receptor (INSR) and suppresses downstream signals, acting as a potent inhibitor of insulin-stimulated MAPK3/ERK1 phosphorylation.
• Signaling Regulation: GRB14 facilitates insulin signal transduction by recruiting PDPK1 to the activated insulin receptor, promoting AKT1 phosphorylation and membrane translocation of PDPK1.
• Subcellular Localization: Predominantly localized in the cytoplasm and associated with endosome membranes, consistent with its role in receptor trafficking and signaling.
• Tissue Distribution: Highly expressed in metabolic and reproductive tissues, including liver, kidney, pancreas, testis, ovary, heart, and skeletal muscle.
• Post‑translational Modifications: Regulated by phosphorylation and acetylation, as identified through proteomic studies; exists in multiple isoforms due to alternative splicing.
• Pathophysiological Relevance: Through its modulation of insulin and growth factor signaling, GRB14 is implicated in metabolic disorders and cancer biology.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1–180 of human GRB14, targeting the N‑terminal region. For Western blot, anticipate a specific band around 61 kDa; consider using reducing conditions and validate the signal in tissue lysates known to express GRB14 (e.g., liver, kidney).
• For ELISA applications, the recombinant protein‑based immunogen suggests compatibility with direct or sandwich ELISA formats; however, optimal coating conditions and detection antibody concentrations should be optimized experimentally.
• The antibody reacts with human, mouse, and rat GRB14; when working with mouse or rat samples, verify that the epitope (aa 1–180) is conserved across species to ensure consistent performance.
• As a polyclonal antibody, it may recognize multiple epitopes; include appropriate controls (e.g., peptide blocking or knockout cell lines) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions, supporting researchers and diagnostic developers from concept to clinic. The anti-GRB14 antibody is part of our portfolio for studying insulin signaling and metabolic pathways; we also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare‑target raw materials to accelerate your assay development.