Anti-GRASP65 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BQQ3

Core Product Specifications and Parameters

Biological Background: GRASP65 (GORASP1) Function and Localization

• GRASP65 (GORASP1, GOLPH5) is a key structural protein of the Golgi apparatus, belonging to the GORASP family. Alternative splicing produces multiple isoforms. (Keywords: Alternative splicing)
• It is required for the formation and maintenance of the Golgi ribbon, acting in concert with GORASP2/GRASP55. It also plays a role in assembly and membrane stacking of cisternae, and in the reassembly of Golgi stacks after mitotic breakdown.
  Related references:
  PMID:33301566
• Caspase-mediated cleavage of GRASP65 is necessary for Golgi fragmentation during apoptosis. (By similarity)
• GRASP65 mediates, via interaction with GOLGA2/GM130, the docking of transport vesicles with Golgi membranes.
  Related references:
  PMID:16489344
• It facilitates ER stress-induced unconventional trafficking of core-glycosylated CFTR to the cell membrane.
  Related references:
  PMID:21884936
• Subcellularly, GRASP65 localizes to the Golgi apparatus, specifically the cis-Golgi network membrane and the endoplasmic reticulum-Golgi intermediate compartment membrane.
• Post-translational modifications include phosphorylation, myristoylation, and zinc-binding. The protein is a phosphoprotein, lipoprotein, and metal-binding protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 221–440 of human GRASP65, located in the C-terminal region. When predicting isoform recognition, consider potential splice variants affecting this domain.
• For Western blotting (WB), validate the antibody in human cell lines; endogenous GRASP65 migrates at approximately 46 kDa. Cleavage fragments may appear during apoptosis—use appropriate controls (e.g., staurosporine-treated cells).
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation (e.g., methanol or paraformaldehyde) and permeabilization to achieve clear Golgi staining. Co-staining with a Golgi marker (e.g., GM130) is recommended.
• For ELISA, the antibody can be used as a capture or detection reagent; titrate to determine optimal concentration for your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined sourcing platform for IVD raw materials, covering every stage from concept to clinic. For GRASP65/Golgi biology studies, we provide validated antibody pairs, customized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Contact us to discuss your specific requirement for rare target raw-material procurement and technical support.