Anti-GRASP65 Monoclonal Antibody for WB, IF/ICC, ELISA - Q9BQQ3

Core Product Specifications and Parameters

Biological Background: GRASP65 Function and Localization

• GRASP65, encoded by the GORASP1 gene, is also known as Golgi peripheral membrane protein p65 or GOLPH5.
• It serves as a key structural protein of the Golgi apparatus, cooperating with GORASP2/GRASP55 to form and maintain the Golgi ribbon. Related references: PMID:33301566
• GRASP65 participates in the assembly and membrane stacking of Golgi cisternae and is critical for the reassembly of Golgi stacks after mitotic breakdown.
• During apoptosis, caspase-mediated cleavage of GRASP65 triggers Golgi fragmentation.
• Through its interaction with GOLGA2/GM130, GRASP65 mediates the docking of transport vesicles at Golgi membranes. Related references: PMID:16489344
• It facilitates ER stress-induced unconventional trafficking of core-glycosylated CFTR to the plasma membrane. Related references: PMID:21884936
• Subcellularly, GRASP65 localizes to the Golgi apparatus, primarily at the cis-Golgi network and ER-Golgi intermediate compartment membranes.
• Post-translational modifications include phosphorylation and myristoylation, reflecting its regulation and membrane association.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band near 46 kDa; validate with appropriate molecular weight markers.
• In immunofluorescence, the antibody should exhibit a Golgi-like staining pattern; optimize fixation and permeabilization conditions.
• The immunogen (aa 341–440) lies in the C-terminal region; cleavage fragments may not be detected; consider using full-length controls.
• For ELISA applications, titrate the antibody to determine optimal coating or detection concentrations.
• Cross-reactivity with human, mouse, and rat has been reported; verify performance in the species of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized recombinant monoclonal antibodies, bulk ancillary reagents, and support for rare target development. Whether you are investigating Golgi dynamics, vesicle trafficking, or cell structure, our one-stop platform streamlines your transition from concept to clinic.