Anti-GRAP Rabbit Polyclonal Antibody for WB and ELISA - Q13588

Core Product Specifications and Parameters

Biological Background: GRAP Function and Localization

• GRAP, also known as GRB2-related adapter protein or DFNB114, is an intracellular signaling adaptor containing SH2 and SH3 domains.
• It couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.
• Plays a critical role in the inner ear and in hearing. Related references: PMID:30610177
• Localizes to the membrane and synapse.
• Associated with non-syndromic deafness; mutations in GRAP are linked to DFNB114 sensorineural hearing loss.
• Belongs to the GRB2 family of adaptor proteins.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 128–217 of human GRAP, which includes the SH2 domain, likely allowing recognition of the full-length protein.
• For Western blot, a band at approximately 25 kDa is expected. Optimize loading and detection conditions for your sample type.
• Suitable for ELISA; perform standard optimization and include appropriate controls.
• Consider testing in relevant cell lines or tissue lysates known to express GRAP, such as neuronal or inner ear samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to validated IVD antibodies like this anti-GRAP polyclonal antibody. From rare target reagents and optimized antibody pairs to bulk ancillary materials, we support your assay development from concept to clinic. Our comprehensive sourcing services ensure reliable supply chains for your Ras signaling and hearing research applications.