Anti-Granzyme B Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P10144

Core Product Specifications and Parameters

Biological Background: Granzyme B Function and Localization

• Granzyme B, also known as C11, CTLA-1, Cathepsin G-like 1 (CTSGL1), Fragmentin-2, and HLP, is a serine protease encoded by the GZMB gene in humans. It is a key component of cytotoxic T-cell and NK cell granules.
• This protease cleaves specifically after aspartic acid residues, a substrate preference shared with caspases, enabling it to activate multiple downstream death pathways. Related references: PMID:1985927, PMID:8258716
• Upon immunological synapse-mediated delivery into target cells, Granzyme B directly cleaves gasdermin-E (GSDME), liberating its pore-forming domain to trigger pyroptosis, a highly inflammatory form of programmed cell death. Related references: PMID:31953257, PMID:32188940
• Granzyme B also proteolytically activates caspase-3, -9, and -10, linking granule exocytosis-mediated cytotoxicity to classical apoptotic execution. Related references: PMID:9852092
• In the context of bacterial infection, Granzyme B can cleave and activate caspase-7 (CASP7) to facilitate plasma membrane repair, a function inferred from sequence similarity.
• Subcellularly, Granzyme B is stored in and secreted from cytolytic granules; it contains an N-terminal signal peptide and undergoes zymogen activation before acquiring full proteolytic activity.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, both of which are critical for proper folding, trafficking, and stability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to human Granzyme B residues 100–200. This likely results in an antibody that recognizes a linear epitope, making it well-suited for denaturing applications such as WB.
• For WB, consider validating the detection of a major band at approximately 28kDa under reducing conditions, corresponding to the zymogen or mature Granzyme B. Processing intermediates may appear at lower molecular weights.
• Given its cross-reactivity with mouse Granzyme B, the antibody can be used in mouse tissue or cell models, but users should validate species-specific performance in their experimental system.
• In IHC-P and IF-P, optimize antigen retrieval and antibody dilution, as the peptide epitope may be sensitive to fixation and embedding conditions.
• For ELISA, direct binding or sandwich formats may require preliminary pairing tests; the polyclonal nature can provide signal amplification but may also increase background if not carefully titrated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories in the apoptosis and immunology sectors by offering this anti-Granzyme B polyclonal antibody as part of a comprehensive IVD raw-materials portfolio. Beyond individual antibodies, we provide validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and custom sourcing for rare targets. Whether you are developing a cytotoxicity assay or pyroptosis-related diagnostic kit, CamelBio streamlines procurement from concept to clinic with a single, reliable B2B partnership.