Anti-Granzyme B Polyclonal Antibody for WB, ELISA - P04187

Core Product Specifications and Parameters

Biological Background: Granzyme B Function and Localization

• Granzyme B (CTLA-1, CCP1, Fragmentin-2) is a serine protease encoded by the Gzmb gene, functioning as a key effector in immune cell-mediated cytotoxicity.
• It is stored in cytolytic granules of cytotoxic T-cells and natural killer (NK) cells and secreted upon immune synapse formation.
• Granzyme B induces caspase-independent pyroptosis by cleaving after aspartic acid residues. Related references: PMID:3555842 PMID:35705808
• It can also trigger pyroptosis via cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety.
• The protease activates caspase-3 and caspase-9 to initiate apoptosis. Related references: PMID:35705808
• It cleaves and activates caspase-7, aiding in plasma membrane repair during bacterial infection. Related references: PMID:35705808
• The protein undergoes proteolytic processing from its zymogen form, contains disulfide bonds, and is glycosylated.
• Keywords: Apoptosis, Cytolysis, Lysosome, Secreted, Serine protease, Hydrolase.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 141–240; therefore, the antibody may recognize both full-length and cleaved forms of Granzyme B.
• For Western blot, a band at approximately 27 kDa is expected in mouse cytotoxic T-cell or NK-cell lysates. Validate with appropriate controls.
• For ELISA, consider using the immunizing peptide as a positive control and test serial dilutions of the antibody.
• Since this is a polyclonal antibody, lot-to-lot variation may occur; include positive and negative controls in every experiment.
• Cross-reactivity with other species is not expected; confirm specificity using mouse Granzyme B knockout samples if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support your immunodiagnostic and research needs. This anti-Granzyme B polyclonal antibody complements our portfolio for apoptosis and immune cytotoxicity studies, enabling robust detection in mouse models. Partner with us to streamline your procurement from concept to clinic.