Anti-Granulin Rabbit Monoclonal Antibody for WB, ELISA - P28799

Core Product Specifications and Parameters

Biological Background: Granulin Function and Localization

• Progranulin (GRN), also known as GP88, PCDGF, proepithelin, and acrogranin, is a secreted glycoprotein belonging to the granulin family.
• Acts as a key regulator of lysosomal function and a growth factor involved in inflammation, wound healing, and cell proliferation (PubMed:12526812, 18378771, 28073925). Related references: PMID:12526812, PMID:18378771, PMID:28073925
• Regulates protein trafficking to lysosomes and the activity of lysosomal enzymes (PubMed:28453791, 28541286). Related references: PMID:28453791, PMID:28541286
• Facilitates lysosomal acidification, causing degradation of mature CTSD by CTSB (PubMed:28073925). Related references: PMID:28073925
• Promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation, preventing release of oxidants and proteases (PubMed:12526812). Related references: PMID:12526812
• Modulates inflammation in neurons by preserving neuron survival, axonal outgrowth, and neuronal integrity (PubMed:18378771). Related references: PMID:18378771
• Additionally, it can inhibit epithelial proliferation and induce IL-8 secretion, and it stabilizes CTSD to maintain its aspartic-type peptidase activity.
• Subcellularly localizes to the lysosome and is secreted; highest tissue expression is observed in kidney, with presence in inflammatory cells and various leukemic cell lines.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region of human Progranulin; consider sequence alignment to assess cross-reactivity with isoforms or orthologs.
• For Western blot, the predicted molecular weight of full-length Progranulin is ~64 kDa, but cleavage products may be observed; optimize sample preparation and validate with appropriate positive controls.
• For ELISA, validate coating concentration and detection conditions in your specific sample matrix to ensure optimal signal-to-noise ratios.
• Cross-reactivity is reported with human and mouse; when using mouse samples, confirm specificity with appropriate controls due to potential endogenous IgG interference in certain assay formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research facilities with a streamlined sourcing platform for IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Granulin monoclonal antibody is part of our portfolio supporting inflammation, oncology, and lysosomal dysfunction research. Partner with CamelBio to access validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and rare-target raw-material sourcing tailored to your development needs.