Anti-GPX8 Rabbit Polyclonal Antibody for WB and ELISA - Q8TED1

Core Product Specifications and Parameters

Biological Background: GPX8 Function and Localization

• GPX8 is a glutathione peroxidase homolog that acts as an endoplasmic reticulum (ER) thiol peroxidase, maintaining ER redox homeostasis and protecting the cytosol from ER-derived H2O2. Related references: PMID:24566470 PMID:32719007
• It functions in a redox relay with ERO1A, using ERO1A-generated H2O2 to drive protein disulfide isomerase (PDI) oxidation, thereby linking peroxide detoxification to productive disulfide formation. Related references: PMID:21215271
• GPX8 modulates ER calcium (Ca2+) levels mainly through SERCA pump activity and partially by influencing passive Ca2+ leakage. Related references: PMID:28129698
• It localizes to the endoplasmic reticulum membrane, consistent with its roles in ER redox and calcium homeostasis.
• As a member of the glutathione peroxidase family, GPX8 contains a conserved catalytic cysteine and exhibits peroxidase activity.
• Keywords include 3D-structure, acetylation, endoplasmic reticulum, isomerase, membrane, oxidoreductase, peroxidase, proteomics identification, reference proteome, transmembrane, transmembrane helix, indicating its structural features and post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 41-209 of human GPX8, targeting the C-terminal region including the peroxidase domain; this design may enhance specificity towards the mature protein.
• For Western blotting, a band at approximately 24 kDa is anticipated; pre-stained molecular weight markers are recommended for size verification.
• When performing ELISA, consider optimizing the coating antigen and antibody dilution to achieve the desired signal-to-noise ratio.
• Given cross-reactivity with mouse and rat, this antibody may be suitable for rodent model studies; however, validation in the specific experimental system is advised.
• Include appropriate positive (e.g., GPX8-overexpressing cell lysate) and negative controls to confirm antibody specificity in your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop partner for IVD raw materials, offering diagnostic manufacturers and research institutes streamlined access to validated antibodies and ancillary reagents. For GPX8-related oxidative stress and ER homeostasis studies, we provide reliable polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing support—helping you accelerate from concept to clinic.