Anti-GPX5 Polyclonal Antibody for WB, ELISA - O75715

Core Product Specifications and Parameters

Biological Background: GPX5 Function and Localization

• GPX5 (epididymal secretory glutathione peroxidase) is also designated EGLP, GPx-5, GSHPx-5, or HEL-S-75p; it belongs to the glutathione peroxidase family.
• The enzyme catalyzes glutathione-dependent reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxides, protecting cells and enzymes from oxidative damage.
• GPX5 is secreted and likely functions in the extracellular milieu, particularly in the epididymal lumen, where it may safeguard sperm membrane lipids from peroxide injury.
• Tissue expression is highly restricted to the epididymis, consistent with a specialized role in sperm maturation and fertility.
• UniProt keywords indicate a known 3D structure, alternative splicing isoforms, and classification as an oxidoreductase/peroxidase.
• The protein contains an N-terminal signal peptide for secretion; the mature polypeptide has a calculated molecular weight of approximately 25 kDa.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal 100 amino acids of human GPX5, which may allow recognition of full-length and certain splice variants; validate detection of isoforms in your system.
• For Western blot, the predicted band size is ~25 kDa; use epididymis tissue lysates or sperm extracts as positive controls and optimize antibody dilution.
• In ELISA, consider coating with recombinant GPX5 or capture from biological fluids; include negative controls to confirm specificity.
• Cross-reactivity with mouse is reported, but verify the expected molecular weight and signal pattern in mouse samples to rule out nonspecific binding.
• Since GPX5 is a secreted protein, testing culture supernatants from relevant cell lines or epididymal fluid may enhance detection sensitivity.
• As a polyclonal reagent, lot-to-lot consistency should be monitored; running a known positive control each time is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. Our anti-GPX5 rabbit polyclonal antibody complements portfolios focused on reproductive health and oxidative stress biomarker development. Beyond this product, we supply validated antibody pairs, bulk recombinant antigens, and ancillary reagents, and provide technical consulting to streamline your assay development and procurement workflow.