Anti-GPX4 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P36969

Core Product Specifications and Parameters

Biological Background: GPX4 Function and Localization

• GPX4 is a phospholipid hydroperoxide glutathione peroxidase that directly reduces phospholipid hydroperoxides in membranes, preventing lipid peroxidation and ferroptosis. (PubMed:40281343, 24439385)
  Related references: PMID:40281343 PMID:24439385
• It also reduces fatty acid-derived hydroperoxides and small soluble peroxides like H2O2, and by similarity cholesterol and thymine hydroperoxides. (PubMed:11115402, 36608588, 17630701)
  Related references: PMID:11115402 PMID:36608588 PMID:17630701
• Primarily expressed in testis, GPX4 is essential for sperm development and male fertility. It is also detected in platelets. (PubMed:11115402)
  Related references: PMID:11115402
• Localized to mitochondria and cytoplasm, GPX4 plays a role in arachidonic acid metabolism in platelets. (PubMed:11115402)
  Related references: PMID:11115402
• GPX4 supports T-cell responses during infection by protecting T-cells from ferroptosis and aiding expansion. (By similarity)
• The selenocysteine active site provides overoxidation resistance, essential for neuronal survival and preventing ferroptosis. (By similarity)
• Post-translational modifications include hydroxylation and phosphorylation; keywords link it to dwarfism and developmental processes.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30-197 of human GPX4, excluding the mitochondrial transit peptide; consider testing across subcellular fractions to confirm isoform recognition.
• For Western blot, the predicted molecular weight is approximately 22 kDa; optimize gel percentage and transfer conditions for low-molecular-weight proteins.
• In immunohistochemical and immunofluorescence applications, include GPX4 knockout controls and validate against known expression patterns (e.g., intense testis staining) or ferroptosis induction models.
• For ELISA, evaluate both direct coating and sandwich formats; use this polyclonal antibody as a detection reagent paired with a GPX4-specific capture antibody.
• Cross-reactivity with mouse and rat is reported; verify reactivity in your experimental system using species-appropriate positive and negative control lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and research laboratories a streamlined channel for high-quality IVD raw materials. For GPX4 research in oxidative stress and ferroptosis, we provide validated polyclonal antibodies, monoclonal antibody pairs, bulk ancillary reagents, and access to rare-target raw materials. Simplify your pipeline from assay concept to clinical application with our integrated sourcing and technical support.