Anti-GPX4 Rabbit Monoclonal Antibody [KD Validated] for WB, IHC, IF, ELISA - P36969

Core Product Specifications and Parameters

Biological Background: GPX4 Function and Localization

• GPX4 is a phospholipid hydroperoxide glutathione peroxidase that directly reduces phospholipid, cholesterol, and thymine hydroperoxides, even when incorporated into membranes and lipoproteins. Related references: PMID:40281343
• It plays a critical role in preventing ferroptosis and protecting cells from oxidative damage by eliminating lipid reactive oxygen species. Related references: PMID:24439385 PMID:40281343
• The active site selenocysteine is essential for oxidative resistance, neuronal survival, and ferroptosis prevention, with Cys substitution resulting in lethality (By similarity).
• Localizes to both mitochondria and cytoplasm, enabling antioxidant function in multiple subcellular compartments.
• Expressed primarily in testis and found in platelets at the protein level. Related references: PMID:11115402
• Involved in arachidonic acid metabolism in platelets and reduces fatty acid-derived hydroperoxides. Related references: PMID:11115402
• Also reduces small soluble hydroperoxides such as H2O2, cumene hydroperoxide, and tert-butyl hydroperoxide. Related references: PMID:17630701 PMID:36608588
• Required for sperm development, photoreceptor cell maturation, and T-cell expansion during immune response (By similarity).

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 74–197 of human GPX4, encompassing the active site selenocysteine region; this design may recognize both mitochondrial and cytoplasmic isoforms.
• For Western blotting, expect bands around 20 kDa and 22 kDa due to alternative initiation or post-translational modifications; validate size shifts using relevant controls.
• In immunofluorescence and immunohistochemistry, consider mitochondrial and cytoplasmic staining patterns; co-localization with organelle markers is recommended for confirmation.
• ELISA performance may vary; titrate antibody and use appropriate blocking to minimize background, especially with platelet or testis lysates.
• Cross-reactivity with mouse and rat GPX4 is supported; however, verify sequence homology in the epitope region for your species of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing solutions for IVD raw materials, including validated antibodies like this anti-GPX4 monoclonal antibody, ideal for oxidative stress and ferroptosis research. With a focus on diagnostic manufacturing and laboratory needs, we provide access to bulk ancillary reagents, optimized antibody pairs, and rare target raw materials. Trust CamelBio as your reliable partner for seamless procurement from discovery to clinical application.