Anti-GPX4 Monoclonal Antibody for WB - P36969

Core Product Specifications and Parameters

Biological Background: GPX4 Function and Localization

• GPX4 (Phospholipid hydroperoxide glutathione peroxidase, also known as GPx-4 or GSHPx-4) is a selenocysteine-containing glutathione peroxidase that directly reduces phospholipid hydroperoxides in membranes and lipoproteins, protecting cells from oxidative damage.
  Related references: PMID:40281343
• It is the key regulator of ferroptosis, an iron-dependent non-apoptotic cell death, by preventing accumulation of lipid reactive oxygen species.
  Related references: PMID:24439385 PMID:40281343
• GPX4 also reduces a broad spectrum of hydroperoxides including cholesterol hydroperoxide, thymine hydroperoxide, fatty acid-derived hydroperoxides, and small soluble peroxides like H₂O₂ and tert-butyl hydroperoxide.
  Related references: PMID:17630701 PMID:36608588 PMID:11115402
• The protein exists as mitochondrial and cytoplasmic isoforms due to alternative initiation; the mitochondrial form contains an N-terminal transit peptide.
• GPX4 is primarily expressed in testis and is crucial for sperm development and male fertility; it is also expressed in platelets and participates in arachidonic acid metabolism.
  Related references: PMID:11115402
• Essential for the maturation and survival of photoreceptor cells, and protects T-cells from ferroptosis during immune responses to infection.
• Keywords associated with GPX4 include antioxidant, peroxidase, oxidoreductase, lipid metabolism, ferroptosis, selenocysteine, alternative initiation, and mitochondrial transit peptide.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 of human GPX4; it may detect both mitochondrial and cytoplasmic isoforms, but confirm reactivity with your specific samples.
• Predicted molecular weight is ~22 kDa; use a suitable SDS-PAGE percentage and validate band identity via knockdown or knockout controls.
• For immunofluorescence (IF-P), permeabilize cells and expect mitochondrial and cytoplasmic staining.
• Cross-reactivity is reported for human, mouse, and rat; always validate in your experimental system.

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