Anti-GPX2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P18283

Core Product Specifications and Parameters

Biological Background: GPX2 Function and Localization

• GPX2 (Glutathione peroxidase 2) is a selenocysteine-containing enzyme belonging to the glutathione peroxidase family. It is also known as gastrointestinal glutathione peroxidase (GPx-GI) or phospholipid hydroperoxide glutathione peroxidase.
• GPX2 catalyzes the reduction of hydroperoxides in a glutathione-dependent manner, regulating cellular redox homeostasis. It can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide, and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides, but not phosphatidylcholine hydroperoxide. Related references: PMID:36608588 PMID:8428933
• The protein is primarily expressed in the liver and gastrointestinal tract, with no significant expression in heart or kidney, indicating a tissue-specific role in antioxidant defense in these tissues.
• Subcellularly, GPX2 is localized in the cytoplasm/cytosol, consistent with its role in intracellular ROS detoxification.
• The active site contains a selenocysteine (Sec) residue, which is essential for its peroxidase activity, and the protein’s molecular weight is approximately 22 kDa.
• Keywords: 3D-structure, oxidoreductase, peroxidase, proteomics identification, reference proteome, selenocysteine.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 100-180 of human GPX2, which may recognize both native and denatured forms; validate in the relevant assay.
• For Western blotting (WB), the predicted molecular weight is ~22 kDa; consider using appropriate gel percentage and transfer conditions.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution for the specific tissue type, especially for gastrointestinal or liver samples given the tissue specificity.
• For ELISA, the antibody may be used as a capture or detection reagent; perform cross-titration experiments to determine the best working concentrations.
• Cross-reactivity with mouse and rat GPX2 has been reported, which allows for multi-species studies; however, confirm with positive controls.

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