Anti-GPX1 Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P07203

Core Product Specifications and Parameters

Biological Background: GPX1 Function and Localization

• Catalyzes glutathione-dependent reduction of hydroperoxides, regulating cellular redox homeostasis. Reduces H2O2, cumene hydroperoxide, tert-butyl hydroperoxide, and fatty acid-derived hydroperoxides (PubMed:11115402, PubMed:36608588). Related references: PMID:11115402 PMID:36608588
• In platelets, GPX1 reduces 12-hydroperoxyeicosatetraenoic acid, a product of the arachidonate 12-lipoxygenase pathway (PubMed:11115402). Related references: PMID:11115402
• Localizes to the cytoplasm and mitochondria.
• Expressed in platelets (at protein level).
• Post-translational modifications include acetylation and phosphorylation; requires selenocysteine for catalytic activity.
• Alternative splicing yields multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen lies within a conserved region (amino acids 100–200), likely contributing to the reported cross-reactivity with mouse and rat GPX1. Researchers using these species should still validate detection in their specific sample type.
• For Western blotting, a band near 22 kDa is expected; run appropriate molecular weight markers and include positive and negative controls.
• For immunohistochemistry (IHC-P), consider testing antigen retrieval methods (heat-induced, citrate buffer pH 6.0) to optimize signal.
• For immunofluorescence (IF/ICC), fixation and permeabilization conditions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering validated GPX1 antibodies, matched antigen pairs, and bulk ancillary reagents for oxidative stress and lipid metabolism research. With a comprehensive portfolio spanning monoclonal and polyclonal antibodies, we help diagnostic developers and researchers accelerate assay development from concept to clinic.