Anti-GPVI/GP6 Rabbit Polyclonal Antibody for WB, ELISA - P0C191

Core Product Specifications and Parameters

Biological Background: GPVI/GP6 Function and Localization

• GPVI (Glycoprotein VI) is a platelet-specific collagen receptor that mediates platelet adhesion and activation upon collagen exposure (PubMed:16139873, PubMed:24368846). Related references: PMID:16139873, PMID:24368846
• It is critical for platelet procoagulant activity, driving thrombin generation and fibrin formation, which contributes to thrombus development in arteries and veins.
• Downstream signaling is transduced via association with the FcR gamma-chain, involving Src family kinases (LYN or FYN), SYK, and adapter protein LAT, ultimately activating PLCG2.
• GPVI localizes to the cell membrane and is expressed exclusively in megakaryocytes and platelets.
• Structurally, it is a transmembrane glycoprotein with immunoglobulin-like domains and disulfide bonds, belonging to the immunoglobulin superfamily (UniProt keywords: Immunoglobulin domain, Repeat, Signal, Transmembrane helix).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 24-265) of mouse GPVI; this region may share sufficient homology with human GPVI to enable the observed cross-reactivity, but validation in human platelet samples is recommended.
• For Western blotting, the predicted molecular weight is ~34 kDa; glycosylation and other post-translational modifications may cause a shift in apparent molecular weight; consider using platelet lysates and appropriate controls.
• In ELISA applications, the antibody may be used for direct detection of recombinant GPVI or for capture/detection in sandwich assays; optimize coating and blocking conditions using mouse or human platelet-derived material.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers and research laboratories by providing reliable access to high-quality IVD raw materials. This anti-GPVI/GP6 antibody supports studies in thrombosis, hemostasis, and platelet biology, and our portfolio extends to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Through our rare-target sourcing and consultative approach, CamelBio accelerates your assay development pipeline from concept to clinical application.