Anti-GPT2 Polyclonal Antibody for WB, ELISA - Q8TD30

Core Product Specifications and Parameters

Biological Background: GPT2 Function and Localization

• GPT2, also known as alanine aminotransferase 2 or glutamate pyruvate transaminase 2, is a pyridoxal phosphate-dependent enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to produce pyruvate and glutamate.
• This reaction plays a central role in amino acid metabolism and the interconversion of carbon skeletons, linking carbohydrate and amino acid metabolic pathways.
• Alternative splicing generates multiple transcript variants, though the primary isoform is widely studied and expressed in key tissues.
• The enzyme is predominantly expressed in muscle, adipose tissue, kidney, and brain, with lower expression in liver and breast, indicating tissue-specific metabolic requirements.
• Post-translational modifications such as acetylation may regulate GPT2 activity or subcellular localization.
• Genetic variants in GPT2 are associated with intellectual disability, underscoring its critical role in neurodevelopment and brain function.
• As a transferase, GPT2 belongs to the class I pyridoxal-phosphate-dependent aminotransferase family, with a well-characterized 3D structure.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 310-410 of human GPT2; consider verifying reactivity with all known isoforms if alternative splicing variants are a concern.
• For western blotting, validate the antibody in cell lines or tissues with expected GPT2 expression, such as muscle, brain, or kidney lysates, and expect a band at approximately 58 kDa.
• When performing ELISA, titrate the antibody and include appropriate controls to establish optimal concentration, as polyclonal antibodies may exhibit lot-to-lot variability.
• Because GPT2 is a pyridoxal phosphate-dependent enzyme, be aware that cofactor availability may affect its conformation and antibody binding in native assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with one-stop access to IVD raw materials, technical services, and consulting—supporting development from concept to clinic. This GPT2 polyclonal antibody serves metabolic and neurological disease research applications, while our comprehensive supply chain offers validated antibody pairs, bulk ancillary reagents, and custom rare-target sourcing. Leverage our expertise to streamline your diagnostic development pipeline.