Anti-GPT2 Mouse Monoclonal Antibody for WB - Q8TD30

Core Product Specifications and Parameters

Biological Background: GPT2/ALT2 Function and Localization

• GPT2, also known as alanine aminotransferase 2 (ALT2) or glutamic-pyruvate transaminase 2, is a pyridoxal phosphate-dependent enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.
• The protein is a member of the class I pyridoxal-phosphate-dependent aminotransferase family and plays a key role in amino acid metabolism and gluconeogenesis.
• GPT2 is highly expressed in muscle, adipose tissue, kidney, and brain, with lower expression in liver and breast, indicating tissue-specific roles in nitrogen metabolism.
• The enzyme localizes predominantly to the mitochondrial matrix, as suggested by its involvement in the malate-aspartate shuttle and mitochondrial alanine metabolism.
• Post-translational modifications include acetylation, and the protein undergoes alternative splicing, generating multiple isoforms that may differ in activity or localization.
• UniProt keywords and annotations highlight its transferase activity, disease association with intellectual disability (MRT49), and availability of 3D structural data for functional studies.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant protein spanning amino acids 358-456 near the C-terminus, which may confer isoform specificity; researchers should verify the relevant isoform in their experimental system.
• For Western blot, consider using tissue lysates from muscle, adipose tissue, kidney, or brain, where GPT2 is highly expressed, and validate with appropriate controls.
• When setting up ELISA, optimize antibody concentrations using recombinant GPT2 protein or cell lysates, and include blocking steps to reduce background.
• Due to alternative splicing, observed band sizes may deviate slightly from the predicted 58kDa; confirm with positive controls or knockdown samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs as a comprehensive IVD raw-material procurement partner, offering validated monoclonal antibodies like this anti-GPT2 clone for metabolism and liver function research. Beyond individual antibodies, we supply optimized antibody pairs, bulk ancillary reagents, and can source rare targets to accelerate your assay development from concept to clinic. Contact us to discuss your specific GPT2-related project needs.