Anti-GPT Rabbit Polyclonal Antibody for WB, ELISA - P24298

Core Product Specifications and Parameters

Biological Background: Alanine Aminotransferase 1 (GPT) Function and Localization

• Alanine aminotransferase 1 (GPT1), also known as glutamate pyruvate transaminase 1, glutamic--alanine transaminase 1, and by the aliases ALT, AAT1, ALT1, SGPT, catalyzes a key transamination reaction.
• Core enzymatic function: reversibly transfers an amino group between alanine and 2-oxoglutarate to form pyruvate and glutamate.
• Metabolic role: participates in cellular nitrogen metabolism and contributes to liver gluconeogenesis by utilizing amino acid precursors transported from skeletal muscles.
• Subcellular localization: predominantly cytoplasmic.
• Tissue expression: highly expressed in liver, kidney, heart, and skeletal muscles; detected at moderate levels in adipose tissue.
• Molecular features: pyridoxal phosphate (vitamin B6)-dependent aminotransferase; subject to acetylation and phosphorylation; identified by direct protein sequencing and mass spectrometry-based proteomics.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1–190) of human GPT. For Western blotting, anticipate a band near 55 kDa and confirm specificity using positive controls (e.g., liver tissue lysates) and appropriate negative controls.
• For ELISA, employ recombinant GPT protein as a standard to optimise coating and detection conditions. Titrate the antibody to achieve the desired sensitivity and dynamic range.
• Stated cross-reactivity includes human, mouse, and rat GPT. However, performance should be verified in your specific sample matrix, as sequence variations may influence binding.
• Post-translational modifications such as acetylation or phosphorylation within or near the immunogen region could affect antibody recognition. If analysing modified GPT, validate the antibody’s binding to the intended form.
• The product is shipped under cold conditions. Upon receipt, store as recommended and minimise freeze-thaw cycles to preserve activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement, providing end-to-end support for diagnostic developers and research laboratories. This GPT polyclonal antibody is suitable for liver function assessment and metabolic disease studies, and we complement it with a broad portfolio of validated antibody pairs, optimised monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing capabilities. Contact CamelBio to simplify your supply chain and advance your projects efficiently.