Anti-GPSM2 Rabbit Polyclonal Antibody for WB, ELISA - P81274

Core Product Specifications and Parameters

Biological Background: GPSM2 Function and Localization

• GPSM2 (G protein-signaling modulator 2), also known as LGN, is a mosaic protein that acts as a guanine nucleotide dissociation inhibitor (GDI) toward G(i) alpha proteins, thereby regulating G protein signaling.
• Through its interaction with NUMA1, GPSM2 plays a pivotal role in mitotic spindle pole organization, ensuring proper chromosome alignment and mitotic progression (PubMed:11781568, PubMed:15632202, PubMed:21816348). Related references: PMID:11781568 PMID:15632202 PMID:21816348
• GPSM2 is required for the recruitment of the cortical dynein-dynactin complex during metaphase, directly influencing spindle orientation and cell polarity (PubMed:22327364). Related references: PMID:22327364
• It is essential for asymmetric cell divisions, a process critical for cell fate determination in development and stem cell maintenance (PubMed:21816348). Related references: PMID:21816348
• Subcellularly, GPSM2 localizes to the cytoplasm, cell cortex, spindle poles, and lateral cell membrane, consistent with its roles in spindle dynamics and cortical signaling.
• Ubiquitously expressed in tissues, GPSM2 is associated with deafness (DFNB82) and encompasses key structural features such as TPR repeats and phosphorylation sites, identified in proteomic studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the central domain of GPSM2 (aa 380-490), providing targeted reactivity; consider epitope accessibility in your specific assay design.
• For Western blotting, use appropriate protein extraction methods and verify the expected molecular weight (~77 kDa) with suitable molecular weight markers; validate band specificity in relevant human or mouse cell lines.
• In ELISA applications, leverage the polyclonal nature for sensitive detection; include appropriate controls to assess cross-reactivity as the antibody reacts with both human and mouse GPSM2.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop platform for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Our expertise spans cell division and signal transduction research, ensuring reliable access to high-quality reagents for diagnostic development. Whether for mitotic spindle studies or asymmetric cell division targets, CamelBio bridges your sourcing needs efficiently.