Anti-GPRC5D Monoclonal Antibody for FC - Q9NZD1

Core Product Specifications and Parameters

Biological Background: GPRC5D Function and Localization

• GPRC5D (G protein-coupled receptor family C group 5 member D) is a member of the GPCR family and is encoded by the GPRC5D gene.
• This receptor is primarily involved in the regulation of hard keratin expression and is believed to play a critical role in the development and maintenance of hair and nails.
• At the cellular level, GPRC5D is localized to the cell membrane, consistent with its function as a transmembrane signal transducer.
• Tissue expression profiling reveals widespread distribution in peripheral tissues: high expression in pancreas; medium in kidney, small intestine, spleen, and testis; low in lung, colon, leukocyte, prostate, and thymus. It is not detected in brain, heart, liver, placenta, skeletal muscle, or ovary.
• The protein undergoes alternative splicing, producing isoforms that may account for the multiple observed molecular weights (34–38 kDa).
• Key structural features include a seven-transmembrane domain topology, typical of G protein-coupled receptors, and the presence of a large N-terminal extracellular domain.
• UniProt keywords highlight its role as a receptor, transducer, and its association with the cell membrane; it has been identified through proteomics and possesses a reference proteome status.

Experimental Guidance and Technical Tips

• This antibody was generated using a synthetic peptide immunogen corresponding to the N-terminal extracellular domain (amino acids 1–100). It may recognize all known isoforms of GPRC5D, but epitope accessibility could vary with conformation or glycosylation.
• For flow cytometry (FC), perform a titration experiment to determine the optimal antibody concentration for your cell type. Use appropriate isotype controls and live/dead discrimination.
• The fluorophore is bright and photostable; minimize light exposure during handling and use freshly prepared samples where possible.
• Given the tissue expression pattern, cell lines or primary cells derived from pancreas, kidney, or immune organs may serve as positive controls; absence in brain or liver can be used for negative control selection.
• If weak signals are observed, consider testing different fixation/permeabilization protocols, as the epitope is located in the N-terminal region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with validated reagents like this anti-GPRC5D monoclonal antibody. Beyond individual antibodies, we offer matched antibody pairs, bulk ancillary reagents, and support for sourcing rare or custom targets. For researchers focused on G protein-coupled receptor biology or keratin expression pathways, CamelBio can streamline your procurement process from concept to clinic.