Anti-GPRC5A Polyclonal Antibody for WB, ELISA - Q8NFJ5

Core Product Specifications and Parameters

Biological Background: GPRC5A Function and Localization

• GPRC5A (Retinoic acid-induced protein 3, also designated RAIG-1, TIG1) belongs to the G protein-coupled receptor family C group 5 and is transcriptionally induced by retinoic acid.
• As an orphan GPCR, GPRC5A is implicated in modulating epithelial cell differentiation and maintaining tissue homeostasis, potentially linking retinoid signals to G protein-mediated pathways.
• The receptor acts as a negative regulator of EGFR signaling, a function observed in related models (By similarity), which may influence cellular proliferation and survival.
• GPRC5A is characterized as a lung tumor suppressor, with its expression frequently silenced or reduced in human lung cancers. Related references: PMID:18000218
• The protein predominantly localizes to the cell membrane and cytoplasmic vesicle membranes, consistent with roles in signal transduction and receptor trafficking.
• Tissue expression is highest in fetal and adult lung, with lower levels detected in kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal cord. Notably, expression is absent or minimal in heart, brain, liver, spleen, thymus, pancreas, skeletal muscle, and peripheral leukocytes. Related references: PMID:18000218 PMID:9857033
• Post-translational modifications include N-linked glycosylation and phosphorylation, which are predicted to regulate receptor stability, localization, and signaling activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 268–357) of human GPRC5A, which may confer specific recognition of the full-length protein and potential cross-reactivity with the mouse ortholog.
• For Western blot, a band around 40 kDa is expected; consider using reducing conditions and appropriate loading controls. Validate detection in GPRC5A-positive cell lines or tissue lysates (e.g., lung).
• ELISA applications can be optimized using recombinant protein or cell-based assays; include proper positive and negative controls to confirm antibody binding characteristics.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio is a dedicated provider of IVD raw materials, offering comprehensive access to antibodies, antigens, and ancillary reagents for diagnostic development. This anti-GPRC5A polyclonal antibody supports research into cancer biology and GPCR signaling, areas where CamelBio can supply matched antibody pairs, bulk reagents, and technical consultation to accelerate your project from concept to clinical application.