Anti-GPR62 Rabbit Polyclonal Antibody for WB, ELISA - Q9BZJ7

Core Product Specifications and Parameters

Biological Background: GPR62 Function and Localization

• GPR62, also known as GPCR8 or KPG_005, is an orphan G protein-coupled receptor encoded by the GPR62 gene (GeneID: 118442) in humans.
• It constitutively activates the G(q/11)/inositol phosphate and the G(s)-alpha/cAMP signaling pathways, and spontaneously recruits beta-arrestin. It also shows reciprocal modulation with the melatonin receptor MTNR1B, likely through receptor heteromerization. Related references: PMID:28827538
• Subcellularly, GPR62 localizes to the cell membrane and endosome membrane.
• Expression is mainly observed in the brain, with detection in the basal forebrain, frontal cortex, caudate, putamen, thalamus, and hippocampus.
• As a GPCR, it possesses transmembrane helices and is predicted to be glycosylated (glycoprotein), which may influence its trafficking and function.
• UniProt categorizes GPR62 under keywords including G protein-coupled receptor, glycoprotein, transmembrane, transducer, and proteomics identification.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide (aa 150-250 of human GPR62, NP_543141.3). In Western blot, a band near 38 kDa is expected; validate in control brain tissue lysates from human, mouse, or rat.
• For ELISA, consider using the immunogen peptide as a positive control to check assay sensitivity and specificity.
• Since the epitope is linear, denaturing and reducing conditions in SDS-PAGE may expose the target epitope. Optimization of sample preparation and antibody dilution is recommended for both WB and ELISA.
• Cross-reactivity is reported for human, mouse, and rat, but testing in the desired species and tissue is advised to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research communities with reliable access to IVD raw materials, including validated antibodies for GPCR targets like GPR62. As a sourcing partner, we offer optimized polyclonal antibodies, matched antibody pairs, and bulk ancillary reagents to support your assay development and basic research needs. Our global supply network ensures consistent delivery of high-quality reagents, helping accelerate your projects from early discovery to clinical applications.