Anti-GPR55 Polyclonal Antibody for WB, IF-P, ELISA - Q9Y2T6

Core Product Specifications and Parameters

Biological Background: GPR55 Function and Localization

• GPR55 is a G protein-coupled receptor (GPCR) that binds lipid ligands such as 2-arachidonoyl lysophosphatidylinositol and lysophosphatidylglucoside with high affinity, triggering intracellular signaling cascades. Related references: PMID:36142844, PMID:36523570, PMID:37544935
• Upon activation, GPR55 couples to GNA13 and RHOA, leading to ERK phosphorylation, Ca2+ release from intracellular stores, and cytoskeletal rearrangements such as stress fiber formation and cell rounding. Related references: PMID:36142844
• In macrophages, GPR55 mediates lysophosphatidylglucoside-induced inhibition of ABCA1 translocation and cholesterol efflux, promoting lipid accumulation and foam cell formation, a process relevant to atherosclerosis. Related references: PMID:37544935
• The receptor is primarily localized to the cell membrane.
• Tissue expression includes the caudate nucleus, putamen, osteoclasts, osteoblasts, and high levels in macrophages and B-cells, but not in hippocampus, thalamus, cerebellum, frontal cortex, or liver. Related references: PMID:36523570, PMID:37544935
• The protein is glycosylated and has a calculated molecular weight of approximately 37 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 210-319 of human GPR55, spanning intracellular and extracellular regions; thus, the antibody may recognize both native and denatured forms. Consider appropriate antigen retrieval or permeabilization protocols for IF-P.
• For Western blot (WB), validate the antibody in your specific sample type, as GPR55 expression may vary. The predicted molecular weight is ~37 kDa, but post-translational modifications may cause bands at different sizes.
• In ELISA and IF-P, ensure proper controls, including knockout or knockdown validation, to confirm specificity, especially given potential cross-reactivity with mouse and rat orthologs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated polyclonal antibodies like this anti-GPR55 antibody, ideal for research in GPCR signaling and lipid metabolism studies. Whether you need optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or assistance in sourcing rare raw materials for challenging targets, CamelBio serves as your reliable partner from concept to clinic.