Anti-GPR35 Rabbit Polyclonal Antibody for WB, ELISA - Q9HC97

Core Product Specifications and Parameters

Biological Background: GPR35 Function and Localization

• GPR35 is a G protein-coupled receptor (GPCR) that functions as a receptor for kynurenic acid, lysophosphatidic acid, and 5-hydroxyindoleacetic acid, mediating diverse signaling pathways. Related references: PMID:16754668, PMID:19473985, PMID:20361937
• Upon ligand binding, it couples with G(i)/G(o) or G(12)/G(13) proteins, leading to inhibition of adenylate cyclase or activation of Rho GTPases, respectively. Related references: PMID:19473985, PMID:35926043, PMID:36543774
• In the cardiovascular system, GPR35 is involved in cardioprotection during ischemia by promoting mitochondrial remodeling through internalization to the outer mitochondrial membrane and inhibiting ATP synthase. Related references: PMID:35926043
• It plays a role in neutrophil recruitment to inflammatory sites and bacterial clearance via the serotonin metabolite 5-HIAA. Related references: PMID:35148838
• GPR35 is predominantly expressed in immune and gastrointestinal tissues.
• The receptor undergoes post-translational modifications including phosphorylation and glycosylation, and contains disulfide bonds essential for its structure.
• Alternative splicing produces multiple isoforms of GPR35, which may exhibit distinct tissue distributions and functions.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider optimizing antibody dilution and using appropriate blocking conditions; the predicted molecular weight is 34 kDa.
• Validate reactivity in your specific tissue or cell line due to potential isoform variability and context-dependent expression.
• This polyclonal antibody may be suitable for detecting GPR35 in mouse samples, as cross-reactivity is indicated, but confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material sourcing, offering this anti-GPR35 polyclonal antibody alongside validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets. Whether advancing GPCR research, investigating immune function, or exploring cardioprotective mechanisms, we provide the procurement support to connect your concept to the clinic efficiently.