Anti-GPR32 Polyclonal Antibody for WB, ELISA - O75388

Core Product Specifications and Parameters

Biological Background: GPR32 Function and Localization

• GPR32 is a probable G protein-coupled receptor, also known as DRV1 and RVDR1.
• It binds resolvin D1 (RvD1) with high affinity, triggering rapid and transient intracellular signaling pathways that enhance macrophage phagocytic and clearance functions. Related references: PMID:20080636
• In macrophages, GPR32 activation reduces migration toward chemoattractants while increasing phagocytosis of microbial particles. Related references: PMID:26969756
• It counter-regulates histamine H1 receptor-induced goblet cell secretion by preventing Ca(2+) increase and ERK1/2 activation via PKC and GRK2. Related references: PMID:23462912
• The receptor is located at the cell membrane, consistent with its transmembrane helix structure and role as a transducer.
• Tissue expression is primarily in resting primary human macrophages, pointing to its role in immune and inflammatory responses.
• Additional structural features include a disulfide bond, glycoprotein modifications, and proteomics identification characteristics, as noted in UniProtKB keywords.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (amino acids 1–100) of human GPR32. Consider verifying batch-specific specificity via peptide competition assays.
• For Western blot, expect a band around 40 kDa. Due to possible glycosylation or other post-translational modifications, minor size shifts may occur; validate in the relevant sample system.
• ELISA applications may require optimization of coating conditions and detection system; consider using the antibody in a sandwich ELISA format if a matched pair is required.
• Reported cross-reactivity with mouse and rat suggests utility in rodent models, but validate endogenous expression levels in the intended experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, delivering reliable supply solutions for antibodies like this anti-GPR32 polyclonal antibody, which supports research in immunology and inflammation. Our extensive portfolio includes validated antibody pairs, bulk ancillary reagents, and rare-target sourcing, all designed to streamline procurement for diagnostic manufacturers and research laboratories from concept to clinical application.