Anti-GPR22 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q99680

Core Product Specifications and Parameters

Biological Background: GPR22 Function and Localization

• GPR22 is an orphan G protein-coupled receptor encoded by the GPR22 gene.
• It signals through a G(i)/G(o) mediated pathway, as shown by functional studies. Related references: PMID:18539757
• GPR22 may be involved in ciliogenesis, based on similarity to other proteins.
• The receptor is localized to the cell membrane, consistent with its seven-transmembrane domain structure.
• GPR22 shows high expression in adult and fetal heart tissue, and is enriched in brain regions including the accumbens, amygdala, cerebellum, cortex, and hippocampus. Related references: PMID:18539757, PMID:9073069
• It is classified under keywords such as cell membrane, cilium biogenesis/degradation, G protein-coupled receptor, glycoprotein, membrane, receptor, transducer, and transmembrane helix.
• The protein has a predicted molecular weight of approximately 49 kDa and undergoes glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain of GPR22, which may allow detection of the receptor in its native membrane-bound form in IF/ICC experiments.
• For Western blotting, a band around 49 kDa is expected; consider using appropriate membrane and blocking conditions to minimize non-specific binding due to the polyclonal nature of the antibody.
• In ELISA, the synthetic peptide immunogen can be used as a positive control to validate binding specificity.
• Cross-reactivity with mouse and rat GPR22 is reported; sequence homology should be verified if using other species.
• Since GPR22 is a GPCR, denaturing conditions in WB may affect epitope recognition; optimization of sample preparation and loading conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw-material sourcing, supporting diagnostic manufacturers and researchers from discovery to clinical application. For orphan GPCR targets like GPR22, we offer validated polyclonal antibodies, custom antibody development, and bulk reagent supply to accelerate your cardiovascular and neuroscience projects. Our global network ensures reliable access to even rare-target raw materials, enabling seamless assay development and scale-up.