Anti-GPR173 Rabbit Polyclonal Antibody for WB and ELISA - Q9NS66

Core Product Specifications and Parameters

Biological Background: GPR173 Function and Localization

• GPR173 (also known as SREB3) is a probable G protein-coupled receptor that belongs to the super conserved receptor expressed in brain (SREB) family.
• It functions as a receptor for the SMIM20-derived peptides phoenixin-14 and phoenixin-20, which are involved in reproductive hormone regulation (By similarity).
• Activation of GPR173 by phoenixin peptides augments gonadotropin-releasing hormone (GNRH) signaling in the hypothalamus and pituitary gland (By similarity).
• In the ovary, GPR173 mediates phoenixin-induced granulosa cell proliferation, promoting follicular growth (PubMed:30933929). Related references: PMID:30933929
• The receptor is localized to the cell membrane, consistent with its role as a transmembrane transducer.
• It is expressed at high levels in the brain, particularly in the cerebellum and cerebral cortex, and at lower levels in the small intestine.
• At the protein level, GPR173 is found in ovarian granulosa cells of follicles beyond the primary stage and in oocytes (PubMed:30933929). Related references: PMID:30933929
• As a GPCR, it contains key structural features such as disulfide bonds, glycosylation sites, and seven transmembrane helices, classifying it as a transducer receptor.

Experimental Guidance and Technical Tips

• The immunogen covers an extracellular region (amino acids 160-230) of GPR173, which may enhance recognition of the native membrane-bound receptor in applications like whole-cell ELISA or immunohistochemistry (if optimized).
• For Western blotting, a band around 41 kDa is expected for the core protein; however, post-translational modifications such as glycosylation may result in a slightly higher apparent molecular weight. Verify in the relevant sample system.
• Given the sequence homology and reported cross-reactivity with mouse, this antibody can potentially be used for studies in mouse models; however, validation in the specific tissue or cell line is recommended.
• As a polyclonal antibody, it may exhibit lot-to-lot variability; include appropriate positive and negative controls, such as tissues known to express high levels (brain, ovary) and knockout controls if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, offering one-stop access to IVD raw materials and technical support from concept to clinic. To accelerate your neuroendocrine and reproductive biology projects, we can supply validated anti-GPR173 antibodies, including polyclonal and monoclonal formats, as well as ancillary reagents and custom antibody development services. Contact us to explore our portfolio and simplify your raw-material procurement.