Anti-GPR17 Rabbit Polyclonal Antibody for WB, ELISA - Q13304

Core Product Specifications and Parameters

Biological Background: GPR17 Function and Localization

• GPR17 is a G protein-coupled receptor that functions as a dual specificity receptor for uracil nucleotides and cysteinyl leukotrienes (CysLTs), signaling primarily through G(i) proteins to inhibit adenylyl cyclase.
• Encoded by the GPR17 gene (Gene ID: 2840), it is also known as the uracil nucleotide/cysteinyl leukotriene receptor, P2Y-like receptor, or receptor R12.
• GPR17 localizes to the cell membrane, typical of GPCRs, where it can interact with extracellular ligands and transduce intracellular signals.
• It is highly expressed in the brain, with additional expression in the kidney, heart, and umbilical vein endothelial cells, suggesting roles in both neural and vascular physiology.
• May mediate brain damage in conditions such as ischemia through the signaling actions of nucleotides and CysLTs, making it a potential target for neuroprotective therapies.
• The receptor exhibits post-translational modifications including glycosylation and disulfide bond formation, characteristic of membrane-bound GPCRs.
• UniProtKB annotation indicates alternative splicing, and its predicted molecular weight is approximately 40,989 Daltons (41 kDa apparent on SDS-PAGE).

Experimental Guidance and Technical Tips

• The immunogen used for this antibody consists of a recombinant fusion protein covering amino acids 1-94 of human GPR17 (extracellular N-terminal region), which may be suitable for detecting the target in Western blot and ELISA across human, mouse, and rat samples.
• Based on the immunogen design targeting the N-terminal region, this antibody may recognize the full-length and potentially glycosylated forms of GPR17. Users are encouraged to validate in their specific sample system with appropriate controls.
• In Western blot, expect a band around 41 kDa; additional bands may appear due to glycosylation or dimerization. For ELISA, coating with recombinant protein or peptide may help verify specificity.
• Reported cross-reactivity with mouse and rat suggests conserved epitopes, but sequence alignment verification is recommended for non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with integrated access to IVD raw materials, including this GPR17 rabbit polyclonal antibody for applications in neurological and cardiovascular disease research. Our comprehensive portfolio covers validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets, supporting your development from early concept to clinical phases.