Anti-GPR155 Polyclonal Antibody for WB, ELISA - Q7Z3F1

Core Product Specifications and Parameters

Biological Background: GPR155 Function and Localization

• GPR155 (Lysosomal cholesterol signaling protein, also known as PGR22, DEPDC3) functions as a cholesterol sensor that couples lysosomal cholesterol levels to mTORC1 signaling. In cholesterol-replete conditions, GPR155/LYCHOS binds cholesterol, disrupting the GATOR1-KICSTOR complex to promote mTORC1 activation; during cholesterol starvation, the GATOR1-KICSTOR interaction is maintained, inhibiting mTORC1. Related references: PMID:36007018 PMID:39358511
• GPR155 also binds indole-3-acetic acid, suggesting a potential role in tryptophan metabolism. Related references: PMID:39358511
• The protein is an integral membrane glycoprotein localized to the lysosome membrane, with multiple transmembrane helices.
• GPR155 is identified as a proteomics target and has a resolved 3D structure (keywords: 3D-structure, Glycoprotein, Lipid-binding, Lysosome, Membrane).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 721-870) of human GPR155, which may confer specificity for the full-length protein and potential cross-reactivity with orthologs in mouse and rat (as indicated).
• For Western blot, the predicted molecular weight is approximately 97 kDa; glycosylation or other post-translational modifications may shift the observed band. Validate using appropriate controls and sample systems.
• ELISA can be employed for quantitative detection; optimization of coating antigen concentration and antibody dilution is recommended.
• As a polyclonal antibody, batch-to-batch consistency should be confirmed in the intended application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, supporting diagnostic developers from concept to clinic. This GPR155 antibody serves research into mTORC1 signaling and cholesterol metabolism, and CamelBio can efficiently source validated pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare-target raw materials to streamline your assay development.