Anti-GPR153 Rabbit Polyclonal Antibody for WB, ELISA - Q6NV75

Core Product Specifications and Parameters

Biological Background: GPR153 Function and Localization

• GPR153 (also known as PGR1) is a probable G protein-coupled receptor and an orphan receptor with no identified endogenous ligand.
• It belongs to the large G protein-coupled receptor (GPCR) superfamily, which are seven-transmembrane domain proteins involved in signal transduction across the cell membrane.
• The receptor is predicted to localize to the cell membrane, consistent with its role as a transmembrane protein.
• GPCRs typically act as transducers for extracellular signals, but the specific signaling pathways activated by GPR153 remain to be elucidated.
• As a reference proteome component identified by proteomics, GPR153 is expressed in human tissues, though its tissue-specific distribution is not fully characterized.
• The protein has a predicted molecular weight of ~65 kDa (UniProt: 65361 Da), aligning with the observed band in Western blot.

Experimental Guidance and Technical Tips

• For Western blot, expect a band at approximately 65 kDa; validate using positive control lysates from human, mouse, or rat tissues.
• When performing ELISA, titrate the antibody to determine optimal working concentration for your specific assay format.
• As GPR153 is an orphan receptor, functional studies may require overexpression systems; consider co-staining with membrane markers to confirm localization.
• Since the immunogen is an N-terminal peptide (amino acids 1-100), the antibody may detect full-length and potential N-terminal variants; verify with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive supply solutions for IVD raw materials, from validated antibodies to ancillary reagents. Our anti-GPR153 polyclonal antibody supports research into orphan GPCRs and signal transduction. Whether you need optimized antibody pairs, bulk reagents, or custom sourcing for rare targets, CamelBio is your reliable partner from concept to clinic.