Anti-GPR151 Polyclonal Antibody for WB, ELISA - Q8TDV0

Core Product Specifications and Parameters

Biological Background: GPR151 Function and Localization

• GPR151 is a member of the G protein-coupled receptor (GPCR) superfamily, also designated as PGR7, GALR4, GALRL, or GPCR-2037.

• It functions as a proton-sensing receptor, transducing extracellular pH changes into intracellular signals.

• Localized primarily to the cell membrane, typical of a transmembrane receptor.

• Predicted structural features include seven-transmembrane helices, disulfide bonds, and glycosylation sites, consistent with GPCR classification.

• High expression observed in the spinal cord, suggesting roles in central nervous system signaling.

• May share functional similarities with galanin receptors, although its endogenous ligand is not galanin.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 310–419) corresponds to the cytoplasmic C-terminal domain; the antibody is expected to recognize both native and denatured protein epitopes.

• For Western blot, a band around 47 kDa is anticipated, though glycosylation may cause slight mobility shifts. Consider treating samples with PNGase F if precise molecular weight confirmation is required.

• The antibody cross-reacts with mouse and rat orthologs; confirm reactivity in the relevant species' spinal cord tissue or cell lines.

• For ELISA, coat the recombinant immunogen or a target peptide; optimize coating concentration and antibody dilution for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, including validated polyclonal and monoclonal antibodies like this anti-GPR151 antibody for neuroscience and GPCR research. Our portfolio encompasses antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support assay development from concept to clinic. Contact us for customized supply solutions tailored to your project needs.