Anti-GPR109A/HM74A/HCAR2 Monoclonal Antibody for WB, ELISA - Q8TDS4

Core Product Specifications and Parameters

Biological Background: GPR109A/HM74A/HCAR2 Function and Localization

• GPR109A, also known as hydroxycarboxylic acid receptor 2 (HCAR2/HM74A), is a G protein-coupled receptor that functions as a high affinity receptor for nicotinic acid (niacin) and (D)-beta-hydroxybutyrate.
• Ligand binding triggers G(i)-protein-mediated inhibition of adenylyl cyclase, leading to reduced intracellular cAMP levels, increased adiponectin secretion, and decreased lipolysis; this pharmacological effect requires nicotinic acid doses far above normal dietary intake.
• The receptor mediates nicotinic acid-induced apoptosis in mature neutrophils by reducing cAMP levels, which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins.
• A rank order of potency for ligand displacement of nicotinic acid binding has been established: 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.
• Subcellular localization is primarily at the cell membrane, consistent with its transmembrane receptor function.
• Tissue expression is largely restricted to adipose tissue and spleen; the receptor is present on mature neutrophils but not on immature neutrophils or eosinophils.
• The receptor belongs to the G protein-coupled receptor family and shares structural features such as disulfide bonds and multiple transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen for this monoclonal antibody is a synthetic peptide within amino acids 100–200; for peptide-based ELISA, consider using the immunogen peptide or a different region as coating antigen to confirm specificity.
• In Western blotting, the predicted molecular weight is approximately 42 kDa; due to post-translational modifications or gel conditions, the observed band may vary slightly.
• As the antibody shows cross-reactivity with mouse and rat, it can be used for homologous target detection in these species, but validation in the relevant sample system is recommended.
• For functional studies involving niacin or ketone body signaling, consider using this antibody to monitor receptor expression levels in adipose tissue, spleen, or neutrophil populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic one-stop partner for IVD raw materials, offering a broad portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support diagnostic development. For researchers focused on metabolic disorders or inflammation, the GPR109A/HCAR2 target is available through our streamlined sourcing pipeline, and we also accommodate rare-target raw-material requests to bridge research concepts to clinical application.