Anti-GPNMB Rabbit Polyclonal Antibody for WB and ELISA - Q14956

Core Product Specifications and Parameters

Biological Background: GPNMB Function and Localization

• GPNMB (Transmembrane glycoprotein NMB), also known as hematopoietic growth factor inducible neurokinin-1 type (HGFIN), is a type I transmembrane protein encoded by the GPNMB gene in humans.
• It is predicted to function as a melanogenic enzyme, potentially involved in melanin biosynthesis, although its catalytic activity remains to be fully defined.
• The protein localizes primarily to the cell membrane and intracellular membranes of melanosomes and early endosomes.
• Post-translational modifications include N-linked glycosylation and phosphorylation, as indicated by proteomic identification.
• GPNMB is widely expressed, with low levels in brain. It is detected in peripheral blood mononuclear cells, tissue macrophages (e.g., Kupffer cells, alveolar macrophages), podocytes, and the ciliary body of the eye. Related references: PMID:12609765 PMID:16609006 PMID:16489096
• In skin, GPNMB is expressed at higher levels in melanocytes compared to keratinocytes and Langerhans cells. Related references: PMID:29336782
• Overexpression of GPNMB has been reported in multiple cancers, including melanoma and glioblastoma multiforme, suggesting a role in tumor progression. Related references: PMID:16489096 PMID:16609006 PMID:7814155
• Genetic variants and alternative splicing have been associated with amyloidosis.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 23–486) of human GPNMB; thus the antibody is expected to recognize the full-length protein in denaturing conditions.
• For western blot, a band migrating at approximately 64 kDa should be observed. Lysates from GPNMB-expressing cell lines (e.g., melanoma) are recommended as positive controls.
• Endogenous GPNMB levels in macrophages or other tissues may be low; consider enrichment strategies or extended exposure times.
• For ELISA, pairing with a matched detection antibody may improve sensitivity for sandwich formats; validate using recombinant protein standards.
• Always include appropriate negative controls (e.g., knockout lysates or tissues with low expression) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For GPNMB-focused oncology or dermatology assay development, we can supply validated antibody pairs, high-titer polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Partner with CamelBio to streamline your workflow and bring robust immunoassays to the next stage.