Anti-GPN1 Rabbit Polyclonal Antibody for WB, ELISA - Q9HCN4

Core Product Specifications and Parameters

Biological Background: GPN1 Function and Localization

• Small GTPase that ensures correct assembly and nuclear import of RNA polymerase II (RNAPII) complex. Related references: PMID:11058119 PMID:20855544 PMID:21768307
• Functions as a chaperone by binding exposed hydrophobic regions of newly synthesized RNAPII subunits, preventing misassembly.
• Acts as an interface between RNAPII and chaperone/scaffolding proteins, linking the polymerase to complex formation regulators. Related references: PMID:17643375
• May facilitate nuclear localization of the DNA repair protein XPA. Related references: PMID:11058119
• Localizes to both the cytoplasm and nucleus.
• Ubiquitously expressed across tissues.
• Subject to post-translational modifications including acetylation and phosphorylation.
• Alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using lysates from human or mouse cell lines known to express GPN1. The expected molecular weight is approximately 42 kDa, though post-translational modifications may cause slight shifts.
• Due to the polyclonal nature of this antibody, optimization of antibody dilution and incubation conditions is recommended for best results.
• For ELISA, validate coating and detection conditions using recombinant GPN1 protein or cell extracts.
• As the antibody cross-reacts with mouse, it can be employed in mouse model studies; verify compatibility with your specific mouse strain.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to IVD raw materials, including validated antibodies like this GPN1 polyclonal antibody for studying RNA polymerase II assembly and transcriptional regulation. Beyond single reagents, we offer one-stop sourcing of antibody pairs, bulk ancillary reagents, and rare target raw materials, supporting your projects from research to clinical application. Contact us to discuss customized procurement solutions and technical consultation.