Anti-GPM6A Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P51674

Core Product Specifications and Parameters

Biological Background: GPM6A Function and Localization

• GPM6A encodes neuronal membrane glycoprotein M6-a, a multi-pass transmembrane protein belonging to the proteolipid protein family.
• The protein is critically involved in neuronal differentiation, promoting migration and differentiation of neuronal stem cells.
• GPM6A regulates neuronal plasticity by mediating neurite and filopodia outgrowth, filopodia motility, and synapse formation through MAPK and Src signaling cascades.
• It is localized to the cell membrane and enriched in specialized neuronal projections, including axons, growth cones, dendritic spines, and filopodia.
• Post-translational modifications include acetylation, palmitoylation, phosphorylation, and N-linked glycosylation; multiple disulfide bonds stabilize its extracellular domains.
• Alternative splicing generates multiple isoforms, and the protein may participate in NGF-dependent calcium influx and intracellular trafficking of GPCRs.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band near 31 kDa. Use reducing conditions and validate with neuronal cell lysates. Include positive and negative controls to confirm specificity.
• For immunohistochemistry (IHC-P), optimize antigen retrieval for your tissue type. GPM6A is highly expressed in brain; consider using cortex or hippocampus sections as positive controls.
• In ELISA, the immunogen peptide can serve as a positive control. Titrate the antibody and consider pairing it with a matched detection antibody for sandwich ELISA development.
• As a polyclonal reagent, verify lot-to-lot consistency for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated access to IVD raw materials, supporting neuronal research with validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our sourcing platform streamlines procurement of rare targets like GPM6A, bridging the gap from early-stage development to clinical application with reliable supply chains and technical consultation.