Anti-GPLD1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P80108

Core Product Specifications and Parameters

Biological Background: GPLD1 Function and Localization

• GPLD1 encodes phosphatidylinositol-glycan-specific phospholipase D (GPI-PLD), a secreted enzyme that hydrolyzes the inositol phosphate linkage of glycosylphosphatidylinositol (GPI)-anchored membrane proteins, releasing them from the cell surface while leaving the phosphate group attached to the membrane. Related references: PMID:17720976 PMID:2760042
• This cleavage can modulate diverse biological processes, including the activation of signaling pathways by releasing GPI-anchored proteins. Related references: PMID:17720976
• The protein is secreted and is involved in lipid metabolism, featuring alternative splicing and glycosylation as indicated by UniProt keywords.
• Its calculated molecular weight is approximately 92.3 kDa, consistent with the observed protein weight of 92 kDa.
• No tissue-specific expression information is available from the provided UniProt record.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 24-160) targets the N-terminal region of human GPLD1, making this antibody suitable for detecting endogenous GPLD1 in human, mouse, and rat samples as per the stated cross-reactivity.
• For Western blotting, consider using appropriate positive and negative controls to confirm specificity for the 92 kDa band.
• In IHC-P and IF/ICC, validate the antibody in the relevant tissue or cell system, as performance may vary depending on fixation and permeabilization conditions.
• As a polyclonal antibody, it may offer enhanced sensitivity due to recognition of multiple epitopes; however, lot-to-lot consistency should be verified for critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your IVD development with this anti-GPLD1 antibody, backed by a comprehensive portfolio of raw materials for GPI anchor biology and signaling research. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents, CamelBio streamlines your supply chain. For rare targets or custom needs, our sourcing network offers a seamless bridge from concept to clinic.