Anti-GPKOW Rabbit Polyclonal Antibody for WB and ELISA - Q92917

Core Product Specifications and Parameters

Biological Background: GPKOW Function and Localization

• GPKOW (G-patch domain and KOW motifs-containing protein) is an RNA-binding protein also designated GPATCH5, MOS2 homolog, and T54.
• It functions in pre-mRNA splicing, specifically as a component of the minor spliceosome that processes U12-type introns.
• The protein localizes to the nucleus, consistent with its role in mRNA processing.
• It contains characteristic G-patch and KOW domains, which are associated with RNA binding and splicing regulation.
• Post-translational modifications include acetylation, phosphorylation, and isopeptide bond formation, potentially modulating its activity.
• UniProt annotations highlight its involvement in mRNA splicing and processing pathways.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 127–476 of human GPKOW, a central region; consider that the antibody may detect splice variants or post-translationally modified forms.
• For Western blotting, validate in appropriate positive control lysates (e.g., HeLa, HEK293) and use a loading control.
• ELISA reactivity suggests suitability for quantitative assays; include appropriate standards and blanks.
• Cross-reactivity with mouse and rat extends utility to rodent models, but endogenous expression levels should be confirmed experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials. This anti-GPKOW antibody supports studies of minor spliceosome function and splicing-related diseases. We offer validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents to streamline your assay development from concept to clinic.