Anti-GPD2 Rabbit Polyclonal Antibody for WB, ELISA - P43304

Core Product Specifications and Parameters

Biological Background: GPD2 Function and Localization

• GPD2 encodes the mitochondrial glycerol-3-phosphate dehydrogenase (mGDH), a flavoprotein-dependent oxidoreductase.
• It plays a key role in the glycerol phosphate shuttle, transferring electrons from cytoplasmic NADH to the mitochondrial electron transport chain.
• The enzyme is calcium-responsive and acts as a critical component of the pancreatic beta-cell glucose-sensing apparatus.
• GPD2 contains a mitochondrial transit peptide and localizes to the mitochondrion.
• Post-translational modifications include phosphorylation and metal binding, as indicated by phosphoprotein and calcium-binding keywords.
• Alternative splicing produces multiple transcript variants, as suggested by UniProt annotation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 25-325) likely excludes the N-terminal mitochondrial targeting sequence, suggesting the antibody may recognize the mature processed form of GPD2.
• For western blotting, a band around 81 kDa is expected; validate specificity using appropriate positive (e.g., pancreatic islets, liver) and negative controls.
• In ELISA, consider optimizing coating conditions and antibody dilution with recombinant protein or tissue lysates from human, mouse, or rat samples.
• Cross-reactivity with mouse and rat enables comparative studies across species; however, confirm reactivity in your specific experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like anti-GPD2 for mitochondrial metabolism and glucose sensing research. Our one-stop sourcing supports everything from small-scale testing to bulk production, covering ancillary reagents and rare target raw material procurement. Explore our offerings to accelerate your diagnostic development from concept to clinic.