Anti-GPD2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P43304

Core Product Specifications and Parameters

Biological Background: GPD2 Function and Localization

• Glycerol-3-phosphate dehydrogenase 2 (GPD2), also known as mitochondrial glycerol-3-phosphate dehydrogenase (mGDH or mtGPD), is encoded by the GPD2 gene; alternative splicing generates multiple isoforms.
• GPD2 functions as a calcium-responsive mitochondrial oxidoreductase, catalyzing the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, a key step in the glycerol phosphate shuttle.
• It plays a critical role in pancreatic beta-cell glucose sensing, coupling glycolysis to mitochondrial ATP production and regulating glucose-stimulated insulin secretion.
• The protein localizes to the mitochondrial inner membrane, consistent with its involvement in mitochondrial energy metabolism.
• GPD2 is a flavoprotein containing FAD as a cofactor and binds calcium, which modulates its enzymatic activity.
• Post-translational modifications include phosphorylation, and the protein is classified as a metal-binding, oxidoreductase reference proteome.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 43-325 of human GPD2, a region that may confer specificity across isoforms; consider epitope mapping if isoform discrimination is needed.
• For western blot (WB), the predicted molecular weight is 81 kDa; ensure appropriate gel percentage and transfer conditions for high-molecular-weight mitochondrial proteins.
• In immunohistochemistry (IHC-P), validate the antibody in your specific tissue type, as mitochondrial proteins may require optimized antigen retrieval methods.
• ELISA performance may depend on the coating buffer and detection system; titration experiments are recommended to establish optimal working dilutions.
• Cross-reactivity with rat is confirmed; for other species, verify sequence homology before applying.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated antibodies like this GPD2 monoclonal antibody for mitochondrial metabolism and diabetes research. Beyond individual reagents, we supply matched antibody pairs, bulk ancillary reagents, and can source rare-target raw materials to accelerate your diagnostic development. Partner with us for reliable, end-to-end support from concept to clinic.