Anti-GPD2 Polyclonal Antibody for WB, ELISA - P43304

Core Product Specifications and Parameters

Biological Background: GPD2 Function and Localization

• GPD2 encodes mitochondrial glycerol-3-phosphate dehydrogenase (mGDH), a flavoprotein oxidoreductase that catalyzes the oxidation of glycerol-3-phosphate to dihydroxyacetone phosphate, feeding electrons into the electron transport chain. It is a calcium-responsive enzyme critical for pancreatic beta-cell glucose sensing and insulin secretion.
• The protein functions as a key component of the glycerol phosphate shuttle, which transfers reducing equivalents from cytosolic NADH to the mitochondrial respiratory chain, linking glycolysis to oxidative phosphorylation.
• mGDH localizes to the mitochondrial inner membrane and contains a transit peptide for mitochondrial targeting, along with a flavin adenine dinucleotide (FAD)-binding domain essential for enzymatic activity.
• Post-translational modifications include phosphorylation, and the protein binds calcium and other metal ions, which modulate its catalytic function.
• Alternative splicing generates multiple isoforms; the canonical isoform has a calculated molecular weight of approximately 81 kDa (80853 Da, UniProt P43304).
• As a member of the glycerol-3-phosphate dehydrogenase family, GPD2 is central to lipid metabolism and energy homeostasis, with implications in diabetes and metabolic disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 25–325 of human GPD2, covering a region downstream of the mitochondrial transit peptide. This design may preferentially recognize the mature, processed form of the protein.
• For Western blot, the predicted molecular weight of GPD2 is ~81 kDa. Consider using whole-cell lysates from pancreatic, hepatic, or muscle tissues, or from relevant cell lines such as INS-1 or HepG2, where mGDH expression is expected.
• Cross-reactivity with mouse and rat is specified; validation in these species is recommended by comparing tissue samples or recombinant protein controls to confirm comparable detection.
• In ELISA applications, titrate both the coating antigen (recombinant protein or tissue lysate) and primary antibody concentration to optimize signal-to-noise ratio. Include controls for matrix effects.
• Because GPD2 activity is calcium-dependent, avoid calcium chelators in lysis or assay buffers unless their effect is experimentally characterized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering a portfolio of validated antibodies, antigen pairs, and ancillary reagents. This anti-GPD2 polyclonal antibody supports research in mitochondrial metabolism and diabetes, and we can further supply matched detection pairs, recombinant proteins, or bulk quantities tailored to your assay development needs. From rare target sourcing to technical consultation, CamelBio accelerates your pathway from concept to clinic.