Anti-GPD1 Rabbit Polyclonal Antibody for WB and ELISA - P21695

Core Product Specifications and Parameters

Biological Background: GPD1 Function and Localization

• GPD1 encodes the cytoplasmic enzyme glycerol-3-phosphate dehydrogenase [NAD(+)], which catalyzes the interconversion of glycerol-3-phosphate and dihydroxyacetone phosphate using NAD+/NADH as cofactors.
• As a key component of the glycerol phosphate shuttle, GPD1 links carbohydrate and lipid metabolism by transferring reducing equivalents from the cytosol to the mitochondrial electron transport chain.
• The enzyme is predominantly expressed in the liver, where it plays a critical role in hepatic gluconeogenesis and triglyceride synthesis.
• Alternative splicing of the GPD1 transcript generates multiple isoforms, potentially expanding functional diversity in different tissues or developmental stages.
• Post-translational phosphorylation of GPD1 has been identified through proteomics analyses, suggesting regulatory modulation of its enzymatic activity.
• Mutations in GPD1 are classified as disease variants, implicating the protein in metabolic disorders; for example, GPD1 deficiency is associated with hypertriglyceridemia.
• Subcellular localization is restricted to the cytoplasm, consistent with its role in cytosolic metabolic pathways.

Experimental Guidance and Technical Tips

• For Western blot, a predominant band near 38 kDa is expected in human, mouse, and rat liver lysates; using a reduced sample may improve resolution.
• Since the antibody is polyclonal, preliminary titration (e.g., 1:500–1:2000) is recommended to optimize signal-to-noise ratio for each application.
• For ELISA, the recombinant immunogen (full-length GPD1) can serve as a positive control to establish assay sensitivity and linearity.
• Given the liver-specific expression, human or rodent liver protein extracts are appropriate positive controls; consider validating in the relevant sample system before large-scale experiments.
• Verify target sequence homology between human and rodent GPD1 if quantitative cross-species comparison is required, although broad cross-reactivity has been confirmed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your IVD development journey by offering a comprehensive range of raw materials, including this anti-GPD1 polyclonal antibody for research on glycerol metabolism and liver biology. Our portfolio extends to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing, ensuring you find the right tools for your assay. Partner with CamelBio for streamlined procurement and technical consultation tailored to your diagnostic needs.