Anti-GPC6 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y625

Core Product Specifications and Parameters

Biological Background: GPC6 Function and Localization

• GPC6, or Glypican-6, is a cell surface proteoglycan that bears heparan sulfate glycosaminoglycan chains, classifying it as a member of the glypican family.
• As a putative coreceptor, GPC6 modulates signaling pathways by interacting with growth factors, extracellular matrix proteins, proteases, and anti-proteases, thereby influencing cellular responses.
• It has been implicated in promoting cancer cell migration and invasion through the WNT5A signaling pathway, highlighting a role in tumor progression.
• GPC6 is localized primarily at the cell membrane via a glycosylphosphatidylinositol (GPI) anchor, but it can also be found secreted into the extracellular space.
• This protein is widely expressed in human tissues, with high levels in fetal kidney and lung, and in adult ovary, liver, kidney, small intestine, and colon; it is also detected in breast cancer cells.
• Key structural features include an N-terminal signal peptide, a GPI-anchor attachment site, and multiple heparan sulfate attachment sites, consistent with its role as a heparan sulfate proteoglycan.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 320–530 of human GPC6 (NP_005699.1), a region likely to be exposed; consider using this antibody in denaturing and native detection methods as indicated by the validated WB and ELISA applications.
• For Western blotting, a band at approximately 63 kDa is expected; verify appropriate loading controls and consider the possibility of post-translational modifications affecting migration.
• In immunofluorescence/ICC, anticipate membrane and possibly extracellular staining; optimize permeabilization and fixation protocols to preserve epitope accessibility.
• Cross-reactivity with mouse GPC6 is reported, making this antibody suitable for studies in mouse models; always validate performance in the specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop sourcing partner for IVD raw materials, offering a comprehensive range of antibodies, antigens, and ancillary reagents. This anti-GPC6 polyclonal antibody supports glycobiology and cancer research, and we can facilitate procurement of validated antibody pairs, bulk polyclonal antibodies, and other rare target raw materials to accelerate your diagnostic development from concept to clinic.