Anti-GPC4 Rabbit Polyclonal Antibody for WB and ELISA - O75487

Core Product Specifications and Parameters

Biological Background: Glypican-4 Function and Localization

• Glypican-4 (GPC4), also known as K-glypican, is a cell surface heparan sulfate proteoglycan.
• It is attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor and can also be found secreted into the extracellular space.
• GPC4 is involved in developmental processes, with functional evidence suggesting roles in kidney tubule morphogenesis and central nervous system development.
• The protein undergoes post-translational modifications including heparan sulfate attachment, glycosylation, and phosphorylation.
• Alternative splicing generates multiple transcript variants, contributing to functional diversity.
• Clinically, mutations or variations in GPC4 have been associated with hearing loss (deafness) and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 357-556) spans the C-terminal region of GPC4, which may include the GPI-anchor attachment domain; consider this when designing detection assays.
• For Western blot, a band around 62kDa is expected, but the molecular weight may shift due to glycosylation and heparan sulfate chains.
• For ELISA, validate the antibody with recombinant protein and native samples to ensure specificity.
• Species cross-reactivity is confirmed with human and mouse; additional validation is recommended for other species.
• When using this antibody in applications beyond the validated WB and ELISA, such as immunohistochemistry, consider performing pilot experiments to optimize conditions.
• As a polyclonal antibody, batch-to-batch consistency should be monitored in critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to validated IVD raw materials, including this anti-GPC4 rabbit polyclonal antibody. Our supply chain offers optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target raw materials, ensuring seamless support from concept to clinic. Partner with CamelBio to accelerate your glypican-4-related research and assay development.