Anti-GPAM Polyclonal Antibody for WB, ELISA - Q9HCL2

Core Product Specifications and Parameters

Biological Background: GPAM Function and Localization

• Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), also known as GPAT1, is the mitochondrial isoform of glycerol-3-phosphate acyltransferase, encoded by the GPAM gene (KIAA1560).
• Catalyzes the first committed step of glycerolipid biosynthesis: esterification of an acyl group from acyl-CoA to the sn-1 position of glycerol-3-phosphate, yielding lysophosphatidic acid (LPA). Related references: PMID:18238778, PMID:19075029, PMID:36522428
• GPAM possesses a narrow hydrophobic binding cleft that selects for linear acyl chains, displaying higher catalytic activity towards substrates with 16-carbon chains. Related references: PMID:36522428
• Resides in the mitochondrial outer membrane, positioning it at a critical hub for lipid synthesis.
• Participates in key metabolic pathways including lipid biosynthesis, phospholipid biosynthesis, and phospholipid metabolism.
• Post-translational modifications such as acetylation and phosphorylation have been identified, potentially modulating enzyme activity.
• A resolved 3D-structure provides detailed structural insights into the catalytic mechanism and substrate specificity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the C‑terminal region (aa 630–730) of human GPAM; sequence alignment across species is recommended to confirm expected reactivity.
• For Western blot, the predicted molecular weight is ~93 kDa. Validate band specificity using appropriate controls in your sample system.
• In ELISA applications, optimize coating and detection conditions empirically, as polyclonal antibodies may exhibit lot‑to‑lot variation.
• Cross‑reactivity with mouse and rat GPAM has been demonstrated; verify performance in those species if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with comprehensive access to IVD raw materials, including validated polyclonal antibodies like this anti‑GPAM product for lipid metabolism studies. Our sourcing network can also supply optimized monoclonal antibodies, antibody pairs, bulk ancillary reagents, and assistance in securing hard‑to‑find raw materials to accelerate your assay development and manufacturing workflows.