Anti-GPA33 Rabbit Monoclonal Antibody - Q99795

Core Product Specifications and Parameters

Biological Background: GPA33 Function and Localization

• GPA33, also known as Cell surface A33 antigen or Glycoprotein A33, is a transmembrane protein with an immunoglobulin domain.
• It may be involved in cell-cell recognition and signaling, though its precise function remains under investigation.
• The protein is localized to the membrane, consistent with its role in cell surface interactions.
• It contains a signal peptide, a disulfide bond, and is subject to palmitoylation, classifying it as a lipoprotein.
• GPA33 is specifically expressed in normal gastrointestinal epithelium and is highly prevalent in colon cancers (95% of cases), making it a marker of intestinal epithelium and a potential therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen is a peptide spanning the C-terminal region (aa 220-319) of human GPA33, which may recognize an epitope accessible in both denaturing and native conditions.
• For Western blot, a band around 35-36 kDa is expected; consider using reducing conditions and suitable loading controls.
• This antibody is suitable for immunohistochemistry (IHC-P) and immunofluorescence (IF-P) on paraffin-embedded tissue sections; antigen retrieval may enhance signal in colon and intestinal tissue samples.
• Given its cross-reactivity with mouse and rat, it may be used in xenograft models or rodent gastrointestinal tissues, but validation in the specific experimental system is recommended.
• ELISA applications may require optimization of coating antigen and antibody concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with streamlined access to IVD raw materials, including this anti-GPA33 rabbit monoclonal antibody for gastrointestinal and oncology research. Our platform supports your workflow by supplying validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, ensuring a seamless path from discovery to clinical application.