Anti-GPA33 Polyclonal Antibody for WB, IF-P, ELISA - Q99795

Core Product Specifications and Parameters

Biological Background: GPA33 Function and Localization

• GPA33 encodes the cell surface A33 antigen, also known as glycoprotein A33, a single-pass type I membrane protein.
• The protein contains an immunoglobulin-like domain and is subject to N-glycosylation and palmitoylation.
• It is proposed to participate in cell-cell recognition and signaling processes within gastrointestinal tissues.
• Subcellularly, GPA33 localizes to the plasma membrane.
• Expression is highly restricted to normal gastrointestinal epithelium and retained in approximately 95% of primary and metastatic colon cancers, marking it as a valuable diagnostic and therapeutic target.
• Key post-translational modifications include disulfide bond formation and palmitate lipid anchoring.
• The observed molecular weight is approximately 36 kDa (calculated 35.6 kDa, with glycosylation).

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 22–235); the antibody is likely to recognize native, cell-surface GPA33, making it suitable for immunofluorescence (IF) and ELISA applications.
• In western blotting (WB), anticipate a band near 36 kDa. Given the presence of disulfide bonds, consider testing both reducing and non-reducing conditions to assess potential oligomeric forms.
• Cross-reactivity with mouse and rat suggests epitope conservation across species. Validate detection in your specific sample system, especially when quantifying across species.
• As a polyclonal antibody, robust detection is often achieved, but for quantitative assays where reproducibility is critical, evaluate lot-to-lot consistency.
• For colorectal cancer studies, confirm GPA33 expression by IF on appropriately fixed cells/tissues, given its membrane localization.

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CamelBio empowers diagnostic manufacturers and researchers with a one-stop platform for IVD raw materials, including validated antibodies like this anti-GPA33 polyclonal antibody for gastrointestinal cancer diagnostic development. Our portfolio spans optimized antibody pairs, high-quality polyclonal and monoclonal reagents, bulk ancillary materials, and rare target sourcing, helping you streamline every stage from concept to clinic.