Anti-GP9 Polyclonal Antibody for WB, IHC-P, ELISA - P14770

Core Product Specifications and Parameters

Biological Background: GP9 Function and Localization

• Platelet glycoprotein IX (GP9), also designated GPIX or CD42a, is a membrane glycoprotein and an essential subunit of the GPIb-V-IX receptor complex on the platelet surface.
• The GPIb-V-IX complex functions as the primary receptor for von Willebrand factor (vWF), mediating vWF-dependent platelet adhesion to exposed subendothelium at sites of vascular injury—a critical initiating step in hemostasis.
• GP9 is thought to facilitate proper membrane insertion and orientation of the GPIb subunit within the complex, thereby supporting overall receptor stability and function.
• It belongs to the leucine-rich repeat (LRR) protein family, characterized by tandem LRR motifs that often mediate protein-protein interactions.
• GP9 is a transmembrane protein localized to the plasma membrane; its single-pass transmembrane domain anchors the complex in the platelet membrane.
• Post-translational modifications include N-linked glycosylation (as a glycoprotein) and the formation of at least one disulfide bond, contributing to structural integrity.
• Mutations in the GP9 gene are associated with Bernard-Soulier syndrome (BSS), an autosomal recessive bleeding disorder characterized by macrothrombocytopenia and defective platelet adhesion.
• The protein participates in key biological processes such as blood coagulation, cell adhesion, and hemostasis, as highlighted by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (amino acids 17-147); the antibody may recognize both native and denatured GP9. For WB, consider testing under reducing and non-reducing conditions.
• For IHC-P, target tissues rich in platelets or megakaryocytes (e.g., bone marrow, spleen) may show positive staining. Always include appropriate negative and positive controls.
• Cross-reactivity with mouse and rat GP9 is reported; verify epitope conservation by sequence alignment if using in vivo models.
• For ELISA, the recombinant protein fragment (aa 17-147) can serve as a positive control to assess antibody binding activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and laboratories with high-quality antibodies like this anti-GP9 polyclonal antibody. Beyond individual research reagents, we offer a broad portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized raw-material sourcing for rare targets in platelet biology, hemostasis, and cardiovascular research. Let us streamline your supply chain from concept to clinic.